From Chemistry Towards Technology Step-By-Step From Chemistry Towards Technology Step-By-Step От химии к технологии шаг за шагом 2782-1900 82261 10.52957/27821900_2022_03_08 Научные статьи Scientific articles Научные статьи Mathematical planning of propionic acid bacteria cultivation Математическое планирование культивирования пропионовокислых бактерий https://orcid.org/0000-0003-4883-5325 Павлов Игорь Николаевич Pavlov Igor N. pawlow-in@mail.ru

кандидат технических наук;

candidate of technical sciences;

 Гора Оксана Николаевна Gora Oksana Nikolaevna ФГБОУ ВО Бийский технологический институт (филиал) ФГБОУ ВПО «Алтайский государственный технический университет им. И. И. Ползунова» Бийск Россия Biysk Technological Institute is a Subsidiary of Polzunov Altai State Technical University Biysk Russian Federation Бийский технологический институт (филиал) Алтайского государственного технического университета им. И.И. Ползунова Biysk Institute of Technology, Polzunov Altai State Technical University 23 09 2022 23 09 2022 3 3 8 16 21 06 2022 12 09 2022 https://chemintech.ru/en/nauka/article/82261/view

На основании результатов собственных исследований и анализа литературных данных предложены условия и состав питательной среды для культивирования пропионовокислых бактерий. В качестве инокулята использован концентрат пропионовокислых бактерий, который содержит клетки селективных штаммов Propionibacterium freudenreichii. В составе питательной среды на постоянном уровне поддерживались концентрации: инокулята - 5% об., хлорида кобальта CoCl2 в дозировке 20 мг/л, а также концентрация гидролизованного молока - 5% об. Варьируемыми в составе питательной среды являлось содержание: дрожжевого автолизата, аскорбиновой кислоты, сульфата аммония, лактозы. Полученную смесь культивировали в термостате 7 суток при температуре 30 °С при рН среды 7,0. Оптимизация процесса культивирования проводилась по методу Бокса - Уилсона. Методом математического планирования построен план полного факторного эксперимента ПФЭ 2n, что позволило оптимизировать питательную среду для культивирования пропионовокислых бактерий с наибольшим приростом биомассы бактерий и накоплением витамина В12. Подобраны оптимальные условия культивирования для накопления биомассы Propionibacterium freudenreichii, при которых максимальное значение биомассы на 5 сутки культивирование составляет 60,5 г/л с высоким содержанием жизнеспособных клеток пробиотических бактерий 12×1012 КОЕ/см3 и максимальным накоплением витамина В12 на 5 сутки культивирование равным 108,1 мкг/мл.

Based on our own research results and analysis of literature data we have proposed conditions and composition of nutrient media for the cultivation of Propionibacterium freudenreichii. As an inoculum we used propionic acid bacteria concentrate, which contains cells of selective strains of Propionibacterium freudenreichii. In the nutrient medium we kept the following concentrations constant: inoculum - 5% vol, cobalt chloride CoCl2 at a dose of 20 mg/l, and hydrolysed milk concentration - 5% vol. The contents of yeast autolysate, ascorbic acid, ammonium sulphate and lactose were varied in the composition of the nutrient medium. We cultivated the resulting mixture in a thermostat for 7 days at 30 °C and a pH of 7.0. The cultivation process was optimized by the Box-Wilson method. Using the method of mathematical planning we designed a full factor experiment FFE 2n, which allowed us to optimize the nutrient medium for propionic acid bacteria with the highest increase in bacterial biomass and accumulation of vitamin B12. We found optimal cultivation conditions for the Propionibacterium freudenreichii biomass accumulation, where the maximum biomass value at day 5 of cultivation was 60.5 g/l with high viable probiotic bacteria cell content of 12×1012 CFU/cm3 and maximum vitamin B12 accumulation at day 5 of cultivation equal to 108.1 µg/ml.

 пропионовокислые бактерии Propionibacterium freudenreichii культивирование оптимизация процесса питательная среда метод Бокса-Уилсона витамин В12 полнофакторный эксперимент propionic acid bacteria Propionibacterium freudenreichii cultivation process optimisation nutrient medium Box-Wilson method vitamin B12 full-factor experiment

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